Review





Similar Products

nbd pc  (Croda International Plc)
90
Croda International Plc nbd pc
Nbd Pc, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14%3A0-06%3A0+nbd+pc/14%3A0-06%3A0+NBD+PC/pm42298172-214-9-49
Average 90 stars, based on 1 article reviews
nbd pc - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
nbd pe  (Croda International Plc)
90
Croda International Plc nbd pe
Nbd Pe, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14%3A0-06%3A0+nbd+pc/14%3A0-06%3A0+NBD+PC/pm39312388-212-8-20
Average 90 stars, based on 1 article reviews
nbd pe - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
810122c  (Croda International Plc)
90
Croda International Plc 810122c
810122c, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14%3A0-06%3A0+nbd+pc/14%3A0-06%3A0+NBD+PC/pmc11383923-31-10-6
Average 90 stars, based on 1 article reviews
810122c - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
chloroform  (Croda International Plc)
90
Croda International Plc chloroform
Workflow for crosslinking and reconstitution of refolded VDAC1 into membrane vesicles for functional assays (A) Lipid stocks in <t>chloroform</t> are dried down in a round bottom flask, resuspended in aqueous buffer, and extruded to produce unilamellar, monodisperse liposomes. (B) Purified VDAC1 is buffer-exchanged to remove Tris and crosslinked using EGS, an amino-reactive covalent crosslinker. (C) Liposomes and crosslinked (or mock-treated) protein samples are treated with detergent and incubated together for 1 h before the overnight addition of Bio-Beads. Resultant proteoliposomes reconstituted with crosslinked or mock-treated VDAC1, and liposomes containing no protein, can be tested for channel and scramblase activity via fluorescence-based assays.
Chloroform, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14%3A0-06%3A0+nbd+pc/14%3A0-06%3A0+NBD+PC/pmc11383923-31-4-6
Average 90 stars, based on 1 article reviews
chloroform - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


Workflow for crosslinking and reconstitution of refolded VDAC1 into membrane vesicles for functional assays (A) Lipid stocks in chloroform are dried down in a round bottom flask, resuspended in aqueous buffer, and extruded to produce unilamellar, monodisperse liposomes. (B) Purified VDAC1 is buffer-exchanged to remove Tris and crosslinked using EGS, an amino-reactive covalent crosslinker. (C) Liposomes and crosslinked (or mock-treated) protein samples are treated with detergent and incubated together for 1 h before the overnight addition of Bio-Beads. Resultant proteoliposomes reconstituted with crosslinked or mock-treated VDAC1, and liposomes containing no protein, can be tested for channel and scramblase activity via fluorescence-based assays.

Journal: STAR Protocols

Article Title: Protocol for the production and reconstitution of VDAC1 for functional assays

doi: 10.1016/j.xpro.2024.103240

Figure Lengend Snippet: Workflow for crosslinking and reconstitution of refolded VDAC1 into membrane vesicles for functional assays (A) Lipid stocks in chloroform are dried down in a round bottom flask, resuspended in aqueous buffer, and extruded to produce unilamellar, monodisperse liposomes. (B) Purified VDAC1 is buffer-exchanged to remove Tris and crosslinked using EGS, an amino-reactive covalent crosslinker. (C) Liposomes and crosslinked (or mock-treated) protein samples are treated with detergent and incubated together for 1 h before the overnight addition of Bio-Beads. Resultant proteoliposomes reconstituted with crosslinked or mock-treated VDAC1, and liposomes containing no protein, can be tested for channel and scramblase activity via fluorescence-based assays.

Article Snippet: NBD-PC, 1 mg/mL in chloroform , Avanti Polar Lipids , Cat#810122C; Cas: 148533-26-0.

Techniques: Membrane, Functional Assay, Liposomes, Purification, Incubation, Activity Assay, Fluorescence